纯化人源骨髓单克隆间充质细胞移植更利于梗死心脏功能恢复Effect of single clonally purified mesenchymal stem cells transplantation on cardiac function after myocardial infarction
张少衡,葛均波,孙爱军,钱菊英,付晗,胡海峰,林谨仪,徐丹令,王克强,邹云增
摘要(Abstract)：
目的通过制备大鼠心肌梗死模型,分别移植经纯化了的人源骨髓单克隆间充质干细胞(SCMSCs)、未纯化的间充质细胞和单个核细胞及外周血单个核细胞,旨在比较SCMSCs移植是否更有利于心脏功能改善。方法应用磁珠分选和极限稀释细胞培养方法获取SCMSCs,利用贴壁培养方法得到未纯化的骨髓间充质细胞,利用梯度密度离心法得到骨髓单个核细胞和外周血单个核细胞。流式细胞仪分析所得细胞特性后,将其移植到梗死心脏。术后1个月,应用血流动力学技术检测大鼠心脏功能,随后取材,以免疫荧光检测移植细胞的分化情况,用碱性磷酸法计算血管密度。结果流式分析结果显示,SCMSCs99%以上表达间充质干细胞标记蛋白,阴性表达造血细胞标记蛋白。功能检测显示,移植SCMSCs心脏收缩功能(LVdP/dtmax)较明显高于其他各组。血管计数显示,SCMSC组血管密度明显高于其他各组。免疫荧光结果显示,移植的SCMSCs向心肌细胞和血管内皮细胞转化效率明显高于其他骨髓细胞,外周血细胞并不发现分化。结论经纯化均一的SCMSCs移植较目前常用的未纯化、不均一的干细胞移植更利于梗死心脏收缩功能恢复。
关键词(KeyWords)： 骨髓祖代细胞;细胞移植;心肌梗死
基金项目(Foundation): “十五”国家科技攻关计划项目(2004BA714B05-2);; 上海市科委优秀学科带头人计划(03XD14010);; 中国博士后基金(KLF101004)等项目资助
作者(Author): 张少衡,葛均波,孙爱军,钱菊英,付晗,胡海峰,林谨仪,徐丹令,王克强,邹云增
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