胡海锋,葛均波,张少衡,黄浙勇,赵岚,孙爱军,钱菊英,付晗,林谨仪,徐丹令,王克强,邹云增,胡凯

• 1: 复旦大学附属中山医院心内科上海市心血管病研究所
• 2:复旦大学附属中山医院心内科上海市心血管病研究所
• 3:复旦大学附属中山医院心内科上海市心血管病研究所
• 4:复旦大学附属中山医院心内科上海市心血管病研究所

摘要(Abstract)：

目的观察梗死区侧支循环对冠状动脉(冠脉)内骨髓干细胞移植术心肌修复疗效的影响。方法结扎猪左前降支建立心肌梗死模型,2周后行冠状动脉造影进行梗死区侧支循环Rentrop评分,取分值为0(R0)或1(R1)的动物各10头,经冠脉内注射骨髓单个核细胞(BMMNCs)或等体积磷酸盐缓冲液(PBS,对照组),随机分为R0+BMT,R0+PBS,R1+单个核细胞移植(BMT)和R1+PBS4组(n=5)。结果梗死后6周,R1+BMT组Rentrop积分显著升高;R0+BMT与R1+BMT两组心功能均明显提高,以R1+BMT组最显著;血管新生和移植细胞数量在R1+BMT组最明显。结论冠脉内移植BMMNCs对梗死心肌有修复作用,而梗死区侧支循环的存在放大其疗效。

关键词(KeyWords)： 细胞移植;心肌梗死;心室功能

Abstract:

Keywords:

基金项目(Foundation): 国家“十五”科技攻关计划项目(2004BA714B05-2);; 上海市科委优秀学科带头人计划(03XD14010);; 中国博士后基金(KLF101004)等项目资助

作者(Author): 胡海锋,葛均波,张少衡,黄浙勇,赵岚,孙爱军,钱菊英,付晗,林谨仪,徐丹令,王克强,邹云增,胡凯

参考文献(References)：

• [1]Wollert KC,Meyer GP,Lotz J,et al.Intracoronaryautologous bone-marrow cell transfer after myocardial infarction:the BOOST randomised controlled clinical trial.Lancet,2004,364:141-148.
• [2]Zhang S,Guo J,Zhang P,et al.Implantation of bone marrowcells upregulated expression of heat shock protein and VEGF in rat infarcted myocardium.Eur J Cardiothorac Surg,2004,25:188-195.
• [3]赵嫣,葛均波,张少衡,等.骨髓干细胞移植治疗心肌梗死最佳时间段的选择.中国临床医学,2005,12:192-195.
• [4]Kamihata H,Matsubara H,Nishiue T,et al.Implantation of bone marrow mononuclear cells into ischemic myocardium enhances collateral perfusion and regional function via side supply ofangioblasts,angiogenic ligands,and cytokines.Circulation,2001,104:1046-1052.
• [5]Kamihata H,Matsubara H,Nishiue T,et al.Improvement of collateral perfusionandregional function byimplantationof peripheral blood mononuclear cells into ischemic hibernating myocardium.Arterioscler Thromb Vasc Biol,2002,22:1804-1810.
• [6]Britten MB,Abolmaali ND,Assmus B,et al.Infarct remodeling after intracoronary progenitor cell treatment in patients with acute myocardial infarction(TOPCARE-AMI):mechanistic insights from serial contrast-enhanced magnetic resonance imaging.Circulation,2003,108:2212-2218.
• [7]Louissaint AJr,Rao S,Leventhal C,et al.Coordinated interaction of neurogenesis and angiogenesisinthe adult songbird brain.Neuron,2002,34:945-960.
• [8]Kobayashi T,Hamano K,Li TS,et al.Enhancement of angiogenesis bythe implantation of self bone marrowcells in a rat ischemic heart model.J Surg Res,2000,89:189-195.
• [9]Rentrop KP,Cohen M,Blanke H,et al.Changes in collateral channel filling immediately after controlled coronary artery occlusion by an angioplasty balloon in human subjects.J Am Coll Cardiol,1985,5:587-592.
• [10]Goodell M,Brose K,Paradis G,et al.Isolation and functional properties of murine hematopoietic stemcells that are replicatingin vivo.J Exp Med,1996,183:1797-1806.
• [11]Breier G,Albrecht U,Sterrer S,et al.Expression of vascular endothelial growth factor during embryonic angiogenesis and endothelial cell differentiation.Development,1992,114:521-532.
• [12]Palmer TD,Willhoite AR,Gage FH.Vascular niche for adult hippocampal neurogenesis.J Comp Neurol,2000,425:479-494.
• [13]Shen Q,Goderie SK,Jin LK,et al.Endothelial cells stimulate self-renewal and expand neurogenesis of neural stem cells.Science,2004,304:1338-1340.