高同型半胱氨酸血症对血管损伤后新生内膜形成的作用及机制Effects and mechanisms of hyperhomocysteinemia on intimal hyperplasia in balloon-injuried rats carotid arteries
陈凤英,郭艳红,施冰,高炜
摘要(Abstract)：
目的研究饮食诱导的高同型半胱氨酸血症(Hcy)对血管损伤后新生内膜形成的作用及机制。方法采用1g(kg·d)L蛋氨酸灌胃制备Hcy大鼠模型。4周后行左颈动脉拉伤,第14天、第28天取材,检测新生内膜的形成、内皮覆盖和平滑肌细胞增殖情况。结果第14天、第28天高蛋氨酸饮食(HHCY)组比对照组新生内膜增生厚度分别增加36%和33%,新生内膜面积分别增加41%和30%,新生内膜面积与中膜面积之比分别增加36%和21%,管腔面积分别减少47%和61%,中膜面积无明显差别。第14天HHCY组内膜、中膜增殖细胞核抗体阳性颗粒百分比是对照组的1.7倍和2.3倍。第14天、第28天HHCY组血管内皮覆盖率比对照组减少52%和31%。结论高同型半胱氨酸血症促使血管损伤后新生内膜形成,促进平滑肌细胞增殖和抑制内皮修复是其致病机制,提示高同型半胱氨酸血症可能是血管成形术后再狭窄发生的重要的危险因素之一。
关键词(KeyWords)： 半胱氨酸;血管内膜;大鼠
基金项目(Foundation):
作者(Author): 陈凤英,郭艳红,施冰,高炜
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