L-精氨酸抑制球囊损伤后大鼠血管内膜增生的机制研究L-arginine attenuates proten expression of related cell cycle regulatory factors in rats after balloon injury
聂晓敏,刘瑞云,黄振文
摘要(Abstract)：
目的 探讨Ｌ－精氨酸抗血管内膜增生的作用机制是否与影响球囊损伤后血管中细胞周期调控基因、细胞周期依赖性激酶２（ＣＤＫ２）、细胞周期蛋白 Ｅ（ＣｙcｌｉｎE）和增殖细胞核抗原（ＰＣＮＡ）的表达有关。方法 21只大鼠随机分为Ｓ组（假手术组），Ｃ组（球囊损伤对照组）及Ｌ组（球囊损伤＋L－精氨酸组）各组 ｎ= ７。术后 １４ｄ采血测血浆一氧化氮（ＮＯ）水平，并处死大鼠取其胸主动脉，测新生内膜面积，采用免疫组化和计算机图像分析法测ＣDK2、ＣｙclｉｎＥ和PCＮＡ的表达水平。结果 Ｃ组的血浆ＮＯ水平明显低于S组，与Ｃ组相比，Ｌ组的血浆N０水平增高（Ｐ＜0．０１），新生内膜面积减少５９．１％（Ｐ＜０．01），ＣＤＫ2、ＣｙcｌｉｎＥ及PCＮＡ的阳性表达指数分别降低３６．１％，４６．３％和７６．２％((Ｐ均＜０．01）。结论 Ｌ-精氨酸抑制球囊损伤后血管中ＣＤＫ2、ＣｙｃlinＥ及ＰＣＮＡ的高表达可能是其抑制血管损伤后内膜增生的分子机制之一。
关键词(KeyWords)： L-精氨酸;球囊损伤;细胞周期调控
基金项目(Foundation): 河南省科技攻关项目（971200100）
作者(Author): 聂晓敏,刘瑞云,黄振文
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