反意寡核苷酸预防血管成形术后再狭窄
陈伯荣,汪丽蕙,朱国英
摘要(Abstract)：
<正> 反意寡核苷酸(AS-ODN)是人工合成的一小段寡核苷酸.通过配对碱基间氢键的作用,它能与核苷酸正链中的特定碱基顺序即靶顺序互补形成双链复合物,从而发挥多种作用,用于治疗目的的AS-ODN能特异地识别靶顺序,并能与其互补的靶顺序结合成稳定的复合物,且在细胞内有较长的半寿期.未经修饰的ODNs仅有结合特异性及足够的亲和力,而经化学修饰的ODNs除上述特点外,还能抵御核酸酶的降解并易经细胞膜为细胞所摄取.有作者认为AS-ODNs的主要作用在翻译水平而不在转录水平,主要通过其短的互补序列作用到靶顺序上,通过碱基配对,形成RNA或RNA-DNA杂交双螺旋,这种杂交双螺旋一旦形成便很快被分解掉.因此,细胞内的这种短的互补序列的RNA越多,被杂交和消耗掉的mRNA就越多,指导蛋白质翻译的mRNA就越少,从而使蛋白质合成的量亦随之越少.
关键词(KeyWords)：
基金项目(Foundation):
作者(Author): 陈伯荣,汪丽蕙,朱国英
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