大鼠主动脉球囊损伤后白细胞介素10的表达The expression of interleukin 10 in rat aorta after balloon angioplasty
夏春芳,霍勇,吴娜琼,薛林,朱国英,唐朝枢
摘要(Abstract)：
目的 观察抗炎因子白细胞介素 10 (IL 10 )在血管成形术后的表达以探讨IL 10在血管损伤反应过程中的意义。方法 采用RT PCR、WesternBlot及免疫组化技术观察了大鼠主动脉球囊损伤后IL 10mRNA水平、蛋白表达及其蛋白分布的动态变化。结果 正常大鼠主动脉无IL 10的表达 ,在球囊损伤后第 1、7和 14天时 ,IL 10均出现转录 ,蛋白的表达量上调 ,生成的IL 10蛋白主要分布在血管内膜及中膜的血管平滑肌细胞 (VSMC)的胞浆和细胞外基质中。结论 大鼠主动脉球囊损伤后IL 10出现高表达 ,提示IL 10可能参与了血管损伤后局部炎症反应的修复过程
关键词(KeyWords)： 白细胞介素10;血管成形术;炎症反应
基金项目(Foundation): 国家自然科学基金 ( 39730 2 2 0 );; 九五国家科技攻关项目 ( 96 90 6 0 2 0 7)
作者(Author): 夏春芳,霍勇,吴娜琼,薛林,朱国英,唐朝枢
参考文献(References)：
- 1BiasucciLM ,LiuzzoG ,BuffonA ,etal.Thevariableroleofinflammationinacutecoronarysyndromesandinrestenosis.SeminIntervCardiol,1999,4:105110.
- 2DeBoerOJ ,vanderWalAC ,BeckerAE .Atherosclerosis,inflammation,andinfection.JPathol,2000,190:237243.
- 3IrfanL ,KailashBandRazzaqueA .Interleukin10:biology,roleininflammationandautoimmunity.AnnAllergyAsthmaImmunol,1997,79:469484.
- 4MallatZ ,BesnardS ,DuriezM ,etal.Protectiveroleofinterleukin10inatherosclerosis.CircRes,1999,85:el724.
- 5FeldmanLJ,AguirreL ,ZiolM ,etal.Interleukin10inhibitsintimalhyperplasiaafterangioplastyorstentimplantationinhypercholesterolemicrabbits.Circulation,2000,101:908916.
- 6XiaCF ,HuoY ,YinH ,etal.Interleukin10inhibitstheratVSMCproliferationandcollagensecretionstimulatedbyangiotensinⅡ.ChinMedSciJ,(submitted).
- 7DaemenMJAP ,LombardiDM ,BosmanFT ,etal.AngiotensinIIinducessmoothmusclecellproliferationinthenormalandratarterialwall.CirRes,1991,68:450455.
- 8F .奥斯伯,R .步伦特,R .E .金斯顿等.精编分子生物学实验指南.北京科学出版社,1998,103150.
- 9MallatZ ,HeymesC ,OhanJ,etal.Expressionofinterleukin10inadvancedhumanatherosclerosisplaques:relationtoinduciblenitricoxidesynthaseexpressionandcelldeath.ArteriosclerThrombVascBiol,1999,19:611616.
- 10UyemuraK ,DemerLL ,CastleSC ,etal.Cross regulatoryrolesofinterleukin(IL)12andIL 10inatherosclerosis.JClinInvest,1996,97:21302138.
- 11PindeskiOslundLJ ,HedrickCC ,OlveraT ,etal.Interleukin10blocksatherosclerosiseventsinvitroandinvivo.ArteriosclerThrombVascBiol,1999,19:28472853.
- 12SelzmanCH ,MclntyreRCJr,ShamesnBD ,etal.Interleukin10inhibitshumanvascularsmoothmuscleproliferation.JMolCellCardiol,1998,30:889896.
- 13SelzmanCH ,MeldrumDR ,CainBS ,etal.Interleukin10inhibitspostinjurytumornecrosisfactor mediatedhumanvascularsmoothmuscleproliferation.JSuryRes,1998,80:352356