甲氨蝶呤抑制兔颈动脉球囊损伤后新生内膜形成The effects of methotrexate on the neointimal formation after balloon injured arteries in rabbits
孟磊,高炜,喻卓,郭艳红,田清平,赵春玉
摘要(Abstract)：
目的 探讨不同剂量甲氨蝶呤 (MTX)对血管损伤后新生内膜形成的影响。方法 制备兔颈动脉球囊损伤模型 ,动物随机分为对照组、MTX 0 5mg/kg、MTX 1 5mg/kg及MTX 5mg/kg组 ,每组 5～ 8只 ,采用肌肉注射方式给药 ,于术后 2周和 4周取材 ,分别进行伊文思染色观察内皮覆盖情况、增殖细胞核抗原 (PCNA)免疫组化染色和定量组织形态学分析。结果 MTX治疗组血管内膜面积、内膜 /中膜面积比值均显著低于对照组 ,内膜面积抑制率达 4 6 %～ 5 6 %,不同剂量MTX之间差异无显著性。内膜及中膜PCNA阳性细胞数目较对照组减少。伊文思蓝染色显示各组间内皮修复程度差异无显著性。结论 MTX能够有效地抑制球囊损伤后血管平滑肌细胞的增殖和新生内膜形成 ,在一定剂量范围内 ,MTX的作用无明显差异。
关键词(KeyWords)： 再狭窄;甲氨蝶呤;内皮,血管
基金项目(Foundation):
作者(Author): 孟磊,高炜,喻卓,郭艳红,田清平,赵春玉
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