三七总皂甙对兔颈动脉内皮剥脱术后c-myc基因表达及血管平滑肌增殖的影响Effects of total spaonins of panax notoginseng on c-myc gene expression and proliferation of rabbit carotid smooth muscle following endothelial removal
夏豪,李庚山,许家璃,蒋锡嘉
摘要(Abstract)：
50只家兔分为内皮剥脱组（BDE，n＝25），药物干预组（BDEP，n＝25），用导管损伤右颈总动脉内皮，左侧为假手术组（S，n＝25）。BDEP组给予三七总皂甙（PNS600mg·kg－1／d）。斑点杂交显影S组未检测出c－myc基因，BDE组术后1h有基因表达，2h达高峰，6h后降至正常。BDEP组与BDE组相比基因表达差异无显著性。3H胸腺嘧啶核苷（3H－TdR）的液闪计数术后36hS组：28±5cpm／μg，BDE组：542±34cpm／μg，BDEP组：56±12cpm／μg；术后76hS组：27±2cpm／μg，BDE组：351±23cpm／μg，BDEP组：30±12cpm／μg。说明PNS对损伤内皮所致的平滑肌增生有抑制作用，其机制不是通过对c－myc基因表达的影响。
关键词(KeyWords)： 三七总皂甙;内皮剥脱;颈动脉;c－myc基因;增殖
基金项目(Foundation):
作者(Author): 夏豪,李庚山,许家璃,蒋锡嘉
参考文献(References)：
- 1 Lin Shnguang,Zhang Xilong, Chen Qiyun, et al. Effect of panax notobinseng spaonins on increased proliferation of cultured aortic smooth muscle cells stimulated by hypercholesterolemic serum. Acta Pharmacological Sinica, 1993,14: 314.
- 2 Clowes AW,Reidy MA,Clowes MM. Kinetics of cellular proliferation after arterial injury I. Smooth muscle growth in the absence of endothelium. Iab Invest, 1989,49: 237-333.
- 3 Chomezynski P, Sacchi H. Signle-step method of RNA isolation by Acid Guanidinium-Thiocyanate-Phenol-Chloroform Extration. Analytical Biochemistry,1987,162:156.
- 4Nishida K,et al. Arterial injury-induced smooth cell proliferation in rats is accompanied by increase in polyamine synthesis and level. Atherosclerosis, 1990,83: 119.
- 5王岚峰,赵进军,黄永麟,等,原癌基困c-fos及c-myc在血管成形术后异常表达的实验研究.中华心血管病杂志,1995,23:217.
- 6 Miano JM,Niksa RRT,Danishefsky VKJ,et al. Rapid communication: Early proto-oncogene expression in rat aortic smooth muscle cells following endothelial removal. Am J Pathol,1990,137: 761.
- 7Nishida KI,Ablko T,Ishihara M , et al. Arterial injury-induced smooth muscle cell proliferation in rats is accompanied by increase in polyamine synthesis and level.Atherosclerosis,1990,83: 119.
- 8石琳,范盘生,吴铃.三七总皂甙升高颈动脉前列腺素I2及降低血小板血栓素A2 的作用.中国药理学报,1990,11:29。
- 9刘乃奎,王雪青,任晓燕.牛磺酸对内皮剥脱后血管平滑肌细胞增生的影响.中华心血管病杂志,1995,23:386。