过表达p27基因抑制血管损伤后新生内膜的形成Overexpression of p27 gene inhibits the neointimal formation after balloon injury in rabbit
田清平,霍勇,郭艳红,孟磊,王贵松,喻卓,吴小兵,高炜
摘要(Abstract)：
目的 探讨p2 7基因转染对血管损伤后新生内膜形成的影响。方法 以腺病毒为载体转染p2 7基因 ,用MTT法观察其对血管平滑肌细胞增殖的影响 ,用流式细胞仪检测细胞周期 ;在兔颈总动脉球囊损伤模型上转染p2 7基因 ,采用免疫组织化学法检测p2 7基因的表达 ,检测其对新生内膜形成的影响。结果 p2 7可明显抑制培养的血管平滑肌细胞增殖 ,细胞停滞于G1期。兔颈总动脉球囊损伤后转染p2 7基因可以减少新生内膜的形成达 2 7 4 3%。结论 以腺病毒为载体转染p2 7基因可以有效地抑制血管平滑肌细胞增殖及血管损伤后新生内膜的形成。
关键词(KeyWords)： 基因,p27;血管内膜
基金项目(Foundation): 北京市自然科学基金资助(7032027);; 教育部教育振兴行动计划特殊专项(九八五工程)资助
作者(Author): 田清平,霍勇,郭艳红,孟磊,王贵松,喻卓,吴小兵,高炜
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