通心络对家兔血管成形术后平滑肌细胞凋亡及Bcl-2和Bax蛋白表达的影响The effect of Tongxinluo on the apoptosis of smooth muscle cell and the protein expressions of the Bcl-2 and Bax after angioplasty in rabbits
刘冬梅 ,刘闺男 ,韩冬云
摘要(Abstract)：
目的观察通心络对血管成形术后家兔平滑肌细胞凋亡及凋亡相关基因Bcl-2和Bax蛋白表达的影响。方法60只家兔随机分为对照组、高脂组及通心络组(每组20只)。通过两次球囊损伤加高脂饮食制备家兔血管成形术模型,再进行4周的通心络治疗观察,以缺口末端标记法(TUNEL)检测凋亡细胞变化,以免疫组化法检测Bcl-2及Bax蛋白表达的变化。结果高脂组家兔血管平滑肌细胞(VSMC)的凋亡较对照组减少,通心络组细胞凋亡明显多于高脂组。高脂组Bcl-2的阳性表达较对照组明显升高,主要分布于内膜及中膜附近;通心络治疗组Bcl-2表达下降,Bcl-2阳性细胞光密度与对照组及高脂组相比差异有统计学意义。通心络组Bax阳性表达显著高于高脂组,主要位于内膜附近。结论通心络具有促进VSMC凋亡,下调Bcl-2蛋白表达及上调Bax蛋白表达的作用。
关键词(KeyWords)： 细胞凋亡;通心络;血管成形术,气囊;原癌基因蛋白质类
基金项目(Foundation):
作者(Author): 刘冬梅 ,刘闺男 ,韩冬云
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