李玉巧,刘志忠,李小波,苏晓叶,吴俊,陈绍良

• 1:南京医科大学附属南京第一医院心内科

摘要(Abstract)：

目的研究骨髓间充质干细胞对球囊损伤的大鼠颈总动脉内皮修复的影响。方法球囊损伤24只SD大鼠颈总动脉,建立动脉内皮损伤模型,随机分为:(1)治疗组12只SD大鼠,球囊损伤颈总动脉即刻注射1mL骨髓间充质干细胞溶液;(2)对照组12只SD大鼠,球囊损伤颈总动脉即刻注射1mL磷酸盐缓冲液。14d及28d后,取颈总动脉标本作组织病理切片,行苏木精伊红及血管内皮生长因子受体-2、α-平滑肌肌动蛋白免疫组化染色。结果用苏木精伊红染色观察新生内膜的厚度,14d及28d治疗组血管内膜增生均较对照组轻(14d时0.57±0.06cm比1.09±0.06cm,P<0.05;28d时0.43±0.09cm比4.72±0.15cm,P<0.05);用血管内皮生长因子受体-2免疫组化染色观察内皮化程度,治疗组血管内皮细胞覆盖程度高于对照组(14d时70.8%±1.3%比20.4%±1.1%,P<0.05;28d时90.2%±1.3%比10.7%±0.4%,P<0.05),治疗组可见血管内皮生长因子受体-2/5-溴脱氧尿核苷双阳性细胞约占血管内皮生长因子受体-2单阳性细胞的50%。α-平滑肌肌动蛋白免疫组化染色观察平滑肌细胞浸润积分,14d时治疗组平滑肌细胞浸润1.5分,对照组2分;28d时两组分别为1分和3分。结论骨髓间充质干细胞可减轻新生内膜增生,加快损伤血管的内皮化进程,减少平滑肌细胞浸润,从而促进球囊损伤的大鼠颈总动脉内皮完整性修复。

关键词(KeyWords)： 间质干细胞;血管内膜;内皮细胞;肌细胞,平滑肌

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 李玉巧,刘志忠,李小波,苏晓叶,吴俊,陈绍良

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