溶血磷脂酸与饮食所致高血脂幼兔主动脉壁病变发展的关系Relationskip between plasma lysophosphatidic acid concentration and the progression of aortic atherosclerotic lesions in diet induced hyperlipidemic infantile rabbits
张宴,李小梅
摘要(Abstract)：
目的探讨血浆溶血磷脂酸(LPA)水平变化与高脂喂养幼兔主动脉粥样硬化(AS)病变发展的关系。方法2月龄家兔随机分成普通饮食组(A组,n=6)与高脂喂养组(B组,n=11)。分别饲喂高脂饲料与普通饲料。动态测定血脂水平、血浆LPA水平、血清一氧化氮(NO)和丙二醛(MDA)水平及超氧化物歧化酶(SOD)活性。另于各个时间点选取B组中兔一只,处死,进行胸主动脉病理学检查。结果高脂喂养1~2周动脉内膜显示微观结构改变。脂质条纹(FS)形成于高脂喂养第4周并进行性加重。血脂水平自高脂喂养1周后即显著升高,以后进行性升高。血浆LPA水平于FS形成前期即显著升高并达到峰值。血清NO,MDA水平及SOD活性均在FS形成中后期发生显著改变。结论测定高脂饮食哺乳动物血浆LPA水平,同时结合血清NO,MDA水平及SOD活性可预警并判定主动脉脂质条纹性病变的严重程度。
关键词(KeyWords)： 动脉粥样硬化;溶血磷脂素类;一氧化氮;丙二醛;超氧化物歧化酶
基金项目(Foundation):
作者(Author): 张宴,李小梅
参考文献(References)：
- [1]宋颖,曲秀芬.溶血磷脂酸与心血管系统疾病.国外医学.心血管疾病分册,2003,30:345-347.
- [2]Xu YJ,Osama A,Praveen Bhugra,et al.Potential role of lysophos-phatidic acid in hypertension and atherosclerosis.Can J Cardiol,2003,19:1525-1536.
- [3]余燕辉,翟湘萍,李智,等.兔高脂蛋白血症模型的建立及动脉粥样硬化斑块形成的方法学.中国药理学通报,1998,14:473-474.
- [4]汪健,卢永才,郭肇铮.高血脂兔过氧化脂质代谢与PGI2/TxA2平衡.中华医学杂志,1987,67:275-276.
- [5]杨永宗,阮长耿,唐朝枢,等.动脉粥样硬化性心血管病———基础与临床.第1版.北京:科学出版社,2004.1.
- [6]Stary HC,Chandler AB,Dinsmore RE,et al.A definition of ad-vanced types of atherosclerotic lesions and a histological classificationof atherosclerosis.Circulation,1995,92:1355-1374.
- [7]Y Stein,O Stein.Does therapeutic intervention achieve slowing ofprogression of Bona Fide regression of atherosclerotic lesions?Arte-rioscler Thromb Vasc Biol,2001,21:183.
- [8]黄世昌,伍期专.溶血磷脂酸与缺血性(心)脑卒中.基础医学与临床,2003,23:1-4.
- [9]Gueguen G,Gaige B,Grevy JM,et al.Structure activity analysis ofthe effects of lysophosphatidic acid on platelet aggregation.Biochem-istry,1999,38:8440-8450.
- [10]Tokumura A,Kanaya Y,Kitahara,et al.Increased formation of ly-sophosphatidic acids by lysophosphalipase D in serum of hypercholes-terolemic rabbits.J Lipid Res,2002,43:307-315.
- [11]Tokumura A,Miyake M,Nishioka Y,et al.Production of lysophos-phatidic acid by lysophospholipase D in human follicular fluids in vitrofertilization patients.Biol Reprod,1999,61:195-199.
- [12]McIntyre TM,Zimmerman GA,Prescott SM.Biologically active oxi-dized phospholipids.J Biol Chem,1999,274:25189-25192.