胰岛素对牛胸主动脉内皮细胞血管内皮生长因子受体表达的影响及其与一氧化氮合酶活性的关系Effect of insulin on expression of VEGF receptor (flt-1、flk-1/KDR) protein in serum-free cultured bovine thoracic aortic endothelial cells and its relation to the NOS3 activation of endothelium
李书国,曾秋棠
摘要(Abstract)：
目的 评价胰岛素对培养的牛胸主动脉内皮细胞血管内皮生长因子 (VEGF)受体flt 1、flk 1 KDR表达的影响及其与一氧化氮合酶 (NOS)的关系。方法 取新生的小牛胸主动脉 ,作血管内皮细胞原代及传代培养 ,取 4 6代培养细胞用于实验 ;应用不同浓度 ( 30mU L、30 0mU L、30 0 0mU L)的胰岛素和NOS抑制剂 (L NAME)干预培养过程 ,4 8h后取培养细胞 ,应用免疫组化法测定flt 1、flk 1 KDR和NOS3的表达水平。结果 不同浓度胰岛素孵育组后内皮细胞flt 1、flk 1 KDR的表达水平差异无显著性 ,L NAME孵育后各组内皮细胞flt 1、flk 1 KDR的表达水平较单纯胰岛素孵育组差异无显著性。结论 胰岛素对内皮细胞flt 1、flk 1 KDR的表达无直接影响 ;内皮细胞NOS3的活性不是内皮细胞VEGF受体flt 1、flk 1 KDR表达的主要影响因素
关键词(KeyWords)： 胰岛素;血管内皮生长因子受体;血管内皮细胞;一氧化氮合酶
基金项目(Foundation):
作者(Author): 李书国,曾秋棠
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