缬沙坦涂层支架对实验兔血管局部内膜增生及血管紧张素Ⅱ2型受体表达的影响The effect of valsartan eluting-stent on the neo-intima and expression of angiotensin Ⅱ type 2 receptor in experimental rabbits
李贵华,王雷,贾三庆,陈晖,李虹伟,赵林,姚道阔,丁荣晶
摘要(Abstract)：
目的评价缬沙坦涂层支架对血管新生内膜及其血管紧张素Ⅱ2型受体(AT2R)表达的影响,探讨缬沙坦涂层支架防治支架内再狭窄的效果及其可能机制。方法采用多层涂布技术制备缬沙坦涂层支架和载体涂层支架。依据腹主动脉置入支架的类型将15只新西兰大白兔分为裸支架组、载体涂层支架组及缬沙坦涂层支架组,每组5只。术前、术后即刻及支架置入3个月后分别行腹主动脉造影,使用定量冠状动脉造影(QCA)软件测量血管直径及其狭窄百分数。3个月后处死实验兔,将支架血管段切片行HE染色,分别测定并比较三组支架血管段的管腔面积、内外弹力膜围绕面积、新生内膜面积及最大内膜厚度;用免疫组化法检测AT2R蛋白质表达,用RT-PCR方法测定AT2RmRNA表达。结果裸支架组、载体涂层支架组、缬沙坦涂层支架组QCA测量的术前、术后即刻及3个月后支架血管段腹主动脉平均直径差异无统计学意义。裸支架组、载体涂层支架组与缬沙坦涂层支架组的平均管腔面积分别为4345548±125822μm2,4302061±167952μm2和5016269±207934μm2;平均新生内膜面积分别为1119635±163503μm2,1135636±136555μm2和441577±74099μm2;平均最大内膜厚度分别为210±30μm,192±21μm和116±12μm。缬沙坦涂层支架组管腔面积最大,最大内膜厚度、新生内膜面积最小。AT2RmRNA在各组都有表达,在缬沙坦涂层支架组显著高于另外两组。免疫组化可见AT2R蛋白的含量在各组具有相同的趋势。结论缬沙坦洗脱支架通过上调AT2R表达,抑制血管内膜增生,可能具有防治支架术后再狭窄的作用。
关键词(KeyWords)： 支架;缬沙坦;受体,血管紧张素;冠状动脉再狭窄;血管内膜;兔
基金项目(Foundation): 北京市自然科学基金(7042021)
作者(Author): 李贵华,王雷,贾三庆,陈晖,李虹伟,赵林,姚道阔,丁荣晶
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