通过刺激自主神经结丛建立持续局灶性心房颤动动物模型Relationship between right atrium ganglionated plexus and atrial fibrillation: A new canine model for sustained focal atrial fibrillation
耿宁,孙英贤,Sunny Po,BenjammineJ.Scherlag
摘要(Abstract)：
目的探讨一种通过刺激自主神经结丛来建立持续局灶性心房颤动(房颤)动物模型的新方法。方法共选取20只雄性杂交狗,随机分为乙酰胆碱(Acetylcholine)组(10只)和卡巴胆碱(Carbachol)组(10只)。用苯巴比妥钠麻醉,行右侧开胸及心包切开手术,暴露出右肺静脉与右心房之间的脂肪垫,将一注射针插入该脂肪垫中,以备注入乙酰胆碱或卡巴胆碱。将多电极导管(CordisWebster公司,美国)缝合在右上肺静脉、右心房、左心房上。分别在给药前后(乙酰胆碱组:10 mmol/L乙酰胆碱0.5 mL;卡巴胆碱组:10 mmol/L卡巴胆碱0.5 mL),于右心房处行程序期前刺激,来测定有效不应期及诱发房颤,刺激强度分别采用2倍、4倍、10倍及20倍起搏域值,如出现房颤,则记录房颤持续最长时间及房颤时心房电活动的平均周期。结果向右心房脂肪垫注射乙酰胆碱或卡巴胆碱后可明显缩短心房有效不应期,增加诱发房颤最大与最小偶联间期之差,延长房颤持续的时间,缩短房颤时心房电活动的平均周期。乙酰胆碱组,未注射乙酰胆碱时,在刺激强度为2倍、4倍、10倍及20倍起搏域值时,在右心房处所测心房有效不应期分别为(116.3±17.8)ms,(102.3±22.7)ms,(91.7±21.9)ms和(80.0±23.0)ms,注入乙酰胆碱后则分别缩短为(103.8±26.1)ms(P>0.05),(94.7±21.9)ms(P<0.05),(74.3±20.0)ms(P<0.01)和(65.5±17.8)ms(P<0.05)。未注射乙酰胆碱或卡巴胆碱时,所诱发房颤均为短阵性房颤,持续时间为(9.7±6.0)s(乙酰胆碱组)和(10.4±8.0)s(卡巴胆碱组),心房平均电活动周期为(122.0±9.0)ms(乙酰胆碱组)和(120.0±8.0)ms(卡巴胆碱组);注入乙酰胆碱后持续时间延长为(596.7±281.0)s(P<0.01),心房平均电活动周期缩短为(76.0±32.0)ms(P<0.05);注入卡巴胆碱后则诱发出频率极快、持续时间更长的房颤,持续时间延长为(2 364.0±1169.0)s(P<0.01),心房平均电活动周期缩短为(39.0±12.0)ms(P<0.01)。结论向狗右心房脂肪垫注射卡巴胆碱可成功建立持续性房颤的动物模型。
关键词(KeyWords)： 心房纤颤;模型,动物;神经结,自主;乙酰胆碱;卡巴胆碱
基金项目(Foundation):
作者(Author): 耿宁,孙英贤,Sunny Po,BenjammineJ.Scherlag
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