外加电场对血管平滑肌细胞生物学行为的影响The change of cellular growth factor receptor and its effect on the proliferation and migration of vascular smooth muscle cells under electrical fields
刘建平,唐波,何国祥
摘要(Abstract)：
目的探讨电场作用下血管平滑肌细胞(VSMCs)膜表面细胞生长因子受体表达的变化及其对细胞增殖迁移的影响。方法通过外加电场干预装置,对体外培养的大鼠主动脉VSMC直流电场干预,间断显微细胞图像记录和分析,研究不同强度电场(EFs)、不同作用时间下VSMCs迁移和细胞形态的变化;采用免疫细胞化学或免疫荧光染色方法检测EFs干预后,与VSMCs迁移密切相关的血小板衍化生长因子受体(PDGFR)、血管紧张素Ⅱ1型受体(AT1R)和2型受体(AT2R)等的表达,研究EFs影响VSMCs增殖及迁移的机制。结果(1)改进的EFs干预装置作用下,VSMCs膜PDGFR表达增加,部分细胞内PDGFR表达上调,呈不对称分布,在EFs阴极面较集中;细胞中AT1R表达亦增加,但无明显不对称分布现象;AT2R表达未见明显改变。(2)150mV/mmEFs长时间作用下,培养的VSMCs有明显的电场趋化性,细胞向阴极迁移的距离明显高于无EFs作用对照组;细胞PCNA表达与对照培养细胞未见明显差异。结论外加直流EFs干预下,大鼠VSMCs发生形状改变和定向迁移。细胞膜向阴极方向伸展,定向迁移依赖于EFs强度,生理强度的直流EFs对VSMCs增殖没有明显影响。EFs作用下,部分细胞生长因子的表达上调和重分布,可能与细胞定向迁移的启动和维持有关。
关键词(KeyWords)： 血管平滑肌细胞;电场;生长因子
基金项目(Foundation): 国家自然科学基金资助项目(30170236)
作者(Author): 刘建平,唐波,何国祥
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