肌肉电转促血管形成素-1基因治疗大鼠下肢血管闭塞病Intramuscular electroporation of angiopoietin-1 gene augments revascularization in the rat ischemic hindlimb
姜宁,高炜,王日胜,朱建健,郭艳红,王建,沈冬,朱国英,陈光慧,汤健
摘要(Abstract)：
目的 观察肌肉电转促血管形成素－１（ａｎｇｉｏｐｏｉｅｔｉｎ－１，Ａｎｇ－１）基因对大鼠下肢血管闭塞病的治疗效果。方法 制作大鼠下肢血管闭塞病模型，通过电转Ａｎｇ－１进行基因治疗，以ＲＴ－ＰＣＲ及免疫组化方法观察 Ａｎｇ－１基因的表达，应用微血管计数、血管造影技术及相关临床指标观察 Ａｎｇ－１基因导入大鼠体内的生物学效应。结果 （１）肌肉电转ｐｃＤ２Ａｎｇ－１基因可在大鼠局部转基因肌肉组织高效表达Ａｎｇ－１；（２）转ｐｃＤ２Ａｎｇ－１基因组大鼠下肢肌肉组织新生血管及侧支循环数目明显多于同期空载质粒对照组。结论 转Ａｎｇ－１基因可促进大鼠局部组织新生血管形成和侧支循环建立，恢复闭塞部位血供，从而有效治疗血管闭塞病。
关键词(KeyWords)： 促血管形成素-1;基因治疗;血管闭塞病
基金项目(Foundation):
作者(Author): 姜宁,高炜,王日胜,朱建健,郭艳红,王建,沈冬,朱国英,陈光慧,汤健
参考文献(References)：
- 1 Judah F, Patricia AD. Blood vessel formation: what is its molecularbasis? Cell, 1996, 87: 1153-l155.
- 2 Jones N, Dumont DJ. Tek/Tie2 signaling: new and old partners.Cancer Metastasis Rev, 2000, 19(l-2): 13-17.
- 3 Chen KH, Zeng W, Ma DL, et al. "Molecular bypass": Theapplication of vascular endothelial growth factor to gene therapy ofexperimental limb ischemia. Cardiovasc Pathob, 1997,2:107-114.
- 4 Werner R. Differentiation of endothelium. FASEB J, 1995,9:926-933.
- 5 Loughna S, Sato TN. A combinatorial role of angiopoietin-1 and orphanreceptor TIEI pathways in establishing vascular polarity during angiogenesis. Mol Cell, 2001,7:233-239.