毛华,杨晓武,杜峰,胥勇,朱晋坤,文美,刘廷筑

• 1:贵阳市第一人民医院心内科

摘要(Abstract)：

目的探讨膜突蛋白(moesin)及moesin抗体与血管内皮损伤相关疾病的临床相关性。方法利用纯化的重组moesin作为抗原建立间接ELISA,在实验中测定抗原最佳包被浓度、抗体最佳稀释浓度及酶标二抗最佳稀释倍数等。采用酶联免疫吸附法检测120例血管内皮损伤相关疾病[急性冠脉综合征(ACS)20例、原发性高血压30例、颈动脉硬化20例、糖尿病20例及高脂血症30例]患者及健康对照组120例健康体检者血清中抗moesin抗体。结果抗moesin抗体在120例血管内皮损伤相关疾病的阳性检出率较高,达25%～70%,显著高于正常对照组的5%(χ2=55.679,P<0.01);其中ACS组阳性检出率最高达70%,明显高于其他疾病组(χ2=11.774,P<0.05),其他各亚型疾病组之间差异无统计学意义(χ2=1.731,P>0.05)。结论抗moesin抗体在血管内皮损伤相关疾病的患者中有较高的检出率,虽然在伴有单一的导致血管内皮损伤危险因素的高血压、高血脂、糖尿病的患者中抗moesin抗体阳性检出率稍低,但在这些危险因素的协同作用下引起的ACS患者中抗moesin抗体阳性检出率较高。提示抗moesin抗体对于诊断血管内皮损伤,作为一个全新的诊断线索有一定的临床价值。

关键词(KeyWords)： 连接蛋白类;抗体;内皮,血管;创伤和损伤

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 毛华,杨晓武,杜峰,胥勇,朱晋坤,文美,刘廷筑

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