硝酸甘油耐受对心肌缺血/再灌注损伤影响的研究Nitroglycerin tolerance aggravates myocardial ischemia reperfusion injury
范谦,张麟,高峰,马新亮,曹雪滨,杨新春
摘要(Abstract)：
目的验证硝酸甘油(GTN)耐受对耐受产生后的心肌缺血/再灌注损伤的影响。方法雄性SD大鼠随机分组,分别接受GTN[600μg/(kg.h)]或生理盐水静脉滴注12h。检验耐受的产生,耐受和对照大鼠接受40min缺血和4h再灌注。检测心肌细胞凋亡(TUNEL法)、心肌坏死面积(Evansblue-TTC染色)和血清肌酸激酶(CK)、乳酸脱氢酶(LDH)活性。结果与单纯心肌缺血/再灌注相比,硝酸甘油耐受引起了再灌注后心肌凋亡比例、心肌坏死面积和血清心肌酶活性的显著增高,而单纯耐受没有对心脏造成明显损伤。结论实验结果提示除了使心血管对硝酸甘油的敏感性降低外,耐受对再灌注心脏存在一种潜在的损伤作用。
关键词(KeyWords)： 硝酸甘油;药物耐受性;心肌缺血;心肌再灌注损伤
基金项目(Foundation):
作者(Author): 范谦,张麟,高峰,马新亮,曹雪滨,杨新春
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