崔斌,丁小涵,赵刚,宋明宝,于世勇,陈剑飞,黄岚

• 1:第三军医大学新桥医院全军心血管内科中心
• 2:兰州军区兰州总医院保健一部

摘要(Abstract)：

目的探讨内皮型一氧化氮合酶(eNOS)基因修饰的内皮祖细胞(EPCs)移植对大鼠颈动脉球囊损伤后新生内膜增生的影响。方法将携带eNOS基因的逆转录病毒载体pMCVeNOS或携带绿荧光蛋白(GFP)的空病毒载体pMCV-GFP分别转染体外培养的EPCs,得到pMCV-eNOS-EPCs和pMCV-GFP-EPCs。分别将转染的pMCV-eNOS-EPCs、pMCV-GFP-EPCs或生理盐水从尾静脉移植至颈动脉内皮损伤大鼠模型。术后14 d采用逆转录-聚合酶链反应(RT-PCR)、蛋白质印迹法、免疫组织化学及苏木素-伊红(HE)染色方法进行检测。结果 eNOS-EPCs组eNOS信使RNA(mRNA)及蛋白表达水平较GFP-EPCs组及对照组显著增高。与对照组比较,球囊损伤后14 d EPCs移植可显著抑制新生内膜的过度增殖,血管腔狭窄程度显著减轻[eNOS-EPCs组(0.58±0.05)比对照组(1.56±0.21),P<0.01;GFP-EPCs组(0.84±0.09)比对照组,P<0.01]。eNOS过表达可进一步增强EPCs的抗增殖效应[eNOS-EPCs组(0.58±0.05)比GFP-EPCs组(0.84±0.09),P<0.05],显著改善内皮依赖性血管舒张反应。结论 eNOS基因修饰可有效促进EPCs对损伤血管内膜的抗增殖作用,显著改善血管内皮功能。

关键词(KeyWords)： 内皮型一氧化氮合酶;基因转染;内皮祖细胞;移植;新生内膜

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(81100149)

作者(Author): 崔斌,丁小涵,赵刚,宋明宝,于世勇,陈剑飞,黄岚

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