大豆苷元对大鼠颈总动脉球囊损伤后内膜增殖的抑制作用Inhibitions of daidzein on intima proliferation of rat common carotid artery injured by balloon
孙海燕,吕树铮,刘舒,王绿娅,陈韵岱
摘要(Abstract)：
目的探讨大豆苷元对大鼠颈总动脉球囊损伤后内膜增殖的影响。方法Wistar鼠共24只,随机分为溶剂对照组、低剂量[50mg/(kg·d)]大豆苷元组和高剂量[100mg/(kg.d)]大豆苷元组,每组各8只;建立颈动脉球囊损伤动物模型。术后5周实验终点放血处死动物,取血清分别测定三酰甘油(TG)、总胆固醇(TC)、尿素氮(BUN)、丙氨酸氨基转移酶(ALT)和丙二醛(MDA);取术侧颈总动脉,切片、苏木精和伊红染色后光镜下观察血管形态并用计算机图像分析系统测量管腔面积、内膜面积和中膜面积。结果与溶剂对照组相比,低剂量组和高剂量组管腔面积均显著增加(三组分别为86893±83452μm2,199419±103352μm2和222324±56154μm2,P<0.01),内膜面积显著减少(三组分别为234370±125697μm2,147651±94731μm2和17651±9473μm2,P<0.01),内膜面积与中膜面积之比值显著降低(三组分别为172.0%±48.0%,41.3%±25.0%和6.7%±2.3%,P<0.01);三组血清生化指标之间差异无统计学意义。结论大豆苷元对大鼠颈总动脉球囊损伤后内膜增殖有一定的抑制作用。
关键词(KeyWords)： 异黄酮类;血管内膜;细胞增殖
基金项目(Foundation):
作者(Author): 孙海燕,吕树铮,刘舒,王绿娅,陈韵岱
参考文献(References)：
- [1]Serruys PW,de Jaegere P,Kiemeneij F,et al.A comparison of balloon-expanda ble stent implantation with balloon angioplasty in patients with coronary artery disease.N Engl J Med,1994,331:489-495.
- [2]Kollum M,Kaiser S,Kinscherf R,et al.Apoptosis after stent implantation compared with balloon angioplasty in rabbits.Role of macrophages.Arterioscler Thromb Vasc Biol,1997,17:2383-2388.
- [3]Schwartz RS,Huber KC,Murphy JG,et al.Restenosis and the proportional neointimal response to coronary artery injury:results in a porcine model.J Am Coll Cardiol,1992,19:267-274.
- [4]Clarkson TB,Anthony MS,Morgan TM.Inhibition of postmeno-pausal atherosclerosis progression:a comparison of the effects of conjugated equine estrogens and soy phytoestrogens.J Clin Endo-crinol Metab,2001,86:41-47.
- [5]Hwang J,Wang J,Morazzoni P,et al.The phytoestrogen equol increases nitric oxide availability by inhibiting superoxide produc-tion:an antioxidant mechanism for cell-mediated LDL modifica-tion.Free Radic Biol Med,2003,34:1271-1282.
- [6]Squadrito F,Altavilla D,Morabito N,et al.The effect of the phytoestrogen genistein on plasma nitric oxide concentrations,en-dothelin-1levels and endothelium dependent vasodilation in post-menopausal women.Atherosclerosis,2002,163:339-347.
- [7]Regar E,Serruys PW,Bode C,et al.Angiographic findings of the multicenter Randomized Study With the Sirolimus-Eluting Bx Velocity Balloon-Expandable Stent(RAVEL):sirolimus-eluting stents inhibit restenosis irrespective of the vessel size.Circula-tion,2002,106:1949-1956.
- [8]Moussa I,Leon MB,Baim DS,et al.Impact of sirolimus-eluting stents on outcome in diabetic patients:a SIRIUS(SIRolImUS-coated Bx Velocity balloon-expandable stent in the treatment of patients with de novo coronary artery lesions)substudy.Circula-tion,2004,109:2273-2278.
- [9]Weissman NJ,Koglin J,Cox da,et al.Polymer-based paclitaxel-eluting stents reduce in-stent neointimal tissue proliferation:a se-rial volumetric intravascular ultrasound analysis from the TAXUS-IV trial.J Am Coll Cardiol,2005,45:1201-1205.
- [10]Guagliumi G,Farb A,Musumeci G,et al.Sirolimus-eluting stent implanted in human coronary artery for16months.Circula-tion,2003,107:1340.
- [11]Pan W,Ikeda K,Takebe M,et al.Genistein,daidzein and gly-citein inhibit growth and DNA synthesis of aortic smooth muscle cells from stroke-prone spontaneously hypertensive rats.J Nutr,2001,131:1154-1158.
- [12]Langan EM3rd,Youkey JR,Elmore JR,et al.Regulation of MAP kinase activity by growth stimuli in vascular smooth muscle.J Surg Res,1994,57:215-220.
- [13]Guo JM,Kang GZ,Xiao BX.Effect of daidzein on cell growth,cell cycle,and telomerase activity of human cervical cancer in vitro.Int J Gynecol Cancer,2004,14:882-888.
- [14]Gercel-Taylor C,Feitelson AK,Taylor DD.Inhibitory effect of genistein and daidzein on ovarian cancer cell growth.Anticancer Res,2004,24:795-800.
- [15]Liu S,Wang L,Wang W,et al.TSC-36/FRP inhibits vascular smooth muscle cell proliferation and migration.Exp Mol Pathol,2006,80:132-140.