雷帕霉素支架对小型猪冠状动脉p27kip表达及内膜增殖的影响The modulation of neointimal proliferation and p27 kip expression after implanting rapamycin-eluting stents in the porcine coronary model
刘学波,葛均波,钱菊英,王克强,贾剑国,姚瑞明,蔡乃绳
摘要(Abstract)：
目的 观察小型猪冠状动脉置入支架后p2 7kip表达变化及含 10 0 μg雷帕霉素可降解涂层支架释放的雷帕霉素对p2 7kip表达的影响。方法 球囊 血管以 1 3∶1比例置入过大的裸支架 (n =14 )、单高分子可降解多聚羟基丙酸乙酸 (PLGA)涂层支架 (n =16 )或雷帕霉素支架 (n =16 )并形成冠状动脉损伤模型 ,术后第 1、2、4和 12周时间段处死部分猪。测定 12周时 3组支架血管段的内膜厚度和面积。免疫组化法测定支架置入后 4个时段冠状动脉的p2 7kip表达水平及动态变化。结果 在 12周的随访组 ,裸支架、PLGA支架和雷帕霉素支架的新生内膜平均厚度分别为 (0 38± 0 2 0 )mm、(0 96± 0 5 8)mm和 (0 14± 0 12 )mm(P =0 0 0 4 ) ,3组的新生内膜面积分别为 (3 38± 0 31)mm2 、(6 4 6±2 0 1)mm2 和 (2 0 7± 0 82 )mm2 (P =0 0 0 0 )。置入裸支架 1周后 ,冠状动脉p2 7kip表达处于低水平状态 ,但在第 4周达到最高水平 ,在第 12周时 ,其表达水平恢复至第 1周时水平 ;雷帕霉素支架使整个随访期的p2 7kip表达水平无明显变化 ,均处于高水平表达状态。结论 含 10 0 μg雷帕霉素支架在 12周内有抑制小型猪冠状动脉内膜增殖的明显疗效。裸支架置入后 12周内 ,p2 7kip表达水平在第 4周最高 ;涂层支架释放的?
关键词(KeyWords)： 雷帕霉素;新生内膜;p27kip
基金项目(Foundation):
作者(Author): 刘学波,葛均波,钱菊英,王克强,贾剑国,姚瑞明,蔡乃绳
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