周音频,黄岚,武晓静,晋军,于仕勇,崔斌,张坡

• 1: 第三军医大学新桥医院全军心血管内科研究所
• 2:第三军医大学新桥医院全军心血管内科研究所
• 3:第三军医大学新桥医院全军心血管内科研究所
• 4:第三军医大学新桥医院全军心血管内科研究所

摘要(Abstract)：

目的探讨在大鼠颈动脉球囊损伤模型中,通过刺激骨髓,加强内皮祖细胞向周围血动员对损伤血管内皮修复和抑制新生内膜的效果。方法制备大鼠颈动脉球囊损伤模型。对照组(n=14)给予生理盐水灌胃,辛伐他汀组(n=12)及合并使用辛伐他汀与粒细胞集落刺激因子(G-CSF)组(n=12)给予辛伐他汀10mg/(kg.d)灌胃,辛伐他汀与G-CSF组加用G-CSF100μg/(kg.d)腹腔注射,给药均从术前1周至术后2周。测定损伤血管内皮修复率、新生内膜/中膜面积比(IA/MA)、增殖性细胞核抗原(PCNA)阳性细胞指数、外周血一氧化氮(NO)含量,流式细胞仪检测CD34+血管内皮生长因子受体2(VEGFR-2)+双荧光阳性细胞比例。结果成功建立大鼠颈动脉损伤模型。与对照组比较,辛伐他汀组内皮修复率增加12.3%,IA/MA减少18.4%、外周血NO含量增加33.9%(P均<0.05);PCNA阳性表达指数降低5.7%(P>0.05);辛伐他汀与G-CSF组上述指标分别为38.2%,50.3%,33.9%和37.2%(P均<0.05)。与对照组比较辛伐他汀可增加外周血CD34+VEGFR-2+双荧光阳性细胞比例87.6%,合用辛伐他汀与G-CSF则可增加达343.4%(P均<0.05)。结论在颈动脉球囊损伤模型,辛伐他汀可以促进损伤血管内皮修复达到抑制新生内膜增殖的效果,其机制可能涉及加强骨髓中内皮祖细胞向外周血的动员。在辛伐他汀基础上合用G-CSF刺激骨髓,可以使上述效应进一步加强。

关键词(KeyWords)： 内皮,血管;粒细胞集落刺激因子;辛伐他汀

Abstract:

Keywords:

基金项目(Foundation): 国家自然基金资助(项目批准号:30470729)

作者(Author): 周音频,黄岚,武晓静,晋军,于仕勇,崔斌,张坡

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