粒细胞集落刺激因子和干细胞因子对急性心肌梗死后心室重构的影响Effects of G-CSF and SCF on ventricular remodeling after acute myocardial infarction
林玲,周胜华,祁述善,沈向前,刘启明,方臻飞
摘要(Abstract)：
目的研究粒细胞集落刺激因子(G-CSF)、干细胞因子(SCF)对急性心肌梗死大鼠心室重构的影响。方法78只大鼠结扎前降支,术后6h尚存活60只。将此60只大鼠随机分为治疗组(30只)和对照组(30只),再将每组分为1,2,4周3个亚组。治疗组皮下注射G-CSF和SCF,对照组皮下注射等体积的生理盐水,计算心室肌质量/体重的比值、心肌梗死面积与左心室肌总面积的百分比,行心肌病理和免疫组化检查。结果(1)心室肌质量/体重的比值:第4周治疗组的比值明显小于对照组(P<0.01);对照组中第4周的比值大于第1周(P<0.01)和第2周(P<0.05);治疗组中第4周的比值大于第1周(P<0·01)。(2)心肌梗死面积与左心室肌总面积的百分比:第2,4周治疗组的面积百分比小于对照组(P<0.05,P<0.01);两组各自组内比较,第4周的面积百分比小于第1,2周(P<0.01)。(3)第4周时HE染色显示治疗组心肌梗死区的心肌较多,而对照组几乎为透壁的疤痕。1~4周治疗组均可见Brdu与TroponinT-I双染阳性的心肌细胞,以第2周时最多,对照组双染阳性细胞少见或偶见。结论G-CSF、SCF治疗大鼠急性心肌梗死可以促使心肌细胞分化,减轻心室重塑。
关键词(KeyWords)： 心肌梗死;细胞因子类;干细胞
基金项目(Foundation):
作者(Author): 林玲,周胜华,祁述善,沈向前,刘启明,方臻飞
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